![]() Online antioxidant post-column bioassay tests were applied to rapidly screen major antioxidants in DOF extracts. Ultra-performance liquid chromatography-electrospray ionisation-quadrupole-time-of-flight-mass spectrometry (UPLC-ESI-QTOF-MS/MS) was performed to investigate the composition of DOF extracts. ![]() Therefore, the present study aimed to investigate the in vitro biological potency of its aqueous extract and screen its active components.Īntioxidant tests, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), the ferric reducing ability of plasma (FRAP), and intracellular reactive oxygen species (ROS) level analyses in primary human epidermal keratinocytes, anti-cyclooxygenase2 (COX-2) assay, anti-glycation assay (both fluorescent AGEs formation in a BSA fructose/glucose system and glycation cell assay), and anti-aging assay (quantification of collagen types I and III, and SA-β-gal staining assay) were conducted to determine the potential biological effects of DOF extracts and its major compounds. However, few studies have reported the well-being beneficial effects of D. ![]() officinale have been extensively studied for active chemical components and various bioactivities. officinale), widely called as "life-saving immortal grass" by Chinese folk, is a scarce and endangered species. Dendrobium officinale Kimura et Migo ( D. ![]()
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